Coding

Part:BBa_K3778026:Design

Designed by: Yun Tsai   Group: iGEM21_CCU_Taiwan   (2021-09-05)


Thrombin releasable TAT48-57


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We modify the last two amino acids, glycine (G) and serine (S), in the thrombin cleavage site to fit the first two amino acids of TAT48-57 (BBa_K3778010) to avoid extra residues at the N-terminal of TAT48-57 after thrombin cleavage.

Source

TAT48-57 is the truncated version of TAT (Trans-Activator of Transcription) protein (BBa_K1202006) modified by CCU_Taiwan team.


References